Recombinant:Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cetuximab Lilly NDC Code: 66733-948-23 Goat-anti-human IgG conjugated with PE Jackson ImmunoResearch Cat#109-116-170, RRID:AB_2337681 PE-conjugated anti-MYC mouse monoclonal antibody Cell Signaling Technology (CST) Cat#3739 Anti-human CD3-BV711 BioLegend Cat#317328, RRID:AB_2562907 Anti-human CD3-BV605 BioLegend Cat#317322, RRID:AB_2561911 Anti-human CD62L-APC BioLegend Cat#304810, RRID:AB_314470 Anti-human CD45RA-BV421 BD Cat#569620 Anti-human LAG3-APC BioLegend Cat#369212, RRID:AB_2728373 Anti-human TIM3-PE-Cy7 BioLegend Cat#345014, RRID:AB_2561720 Anti-human PD1-BV421 BioLegend Cat#329920, RRID:AB_10960742 GPC2 (CT3) antibody CST Cat#90488 Anti-Phospho-PLCγ1 CST Cat#2821S Anti-PLCγ1 CST Cat2822S Anti-Phospho-SLP76 CST Cat#14745S Anti-SLP76 CST Cat#4958S Anti-Phospho-LAT CST Cat#3584S Anti-LAT CST Cat#45533S Anti-Phospho-ZAP70 CST Cat#2701S Anti-ZAP70 CST Cat#2705S Anti-Phospho-mTOR CST Cat#5536 Anti-mTOR CST Cat#2983 Anti-GAPDH CST Cat#2118S Anti-Histone H4 CST Cat#2592S Anti-NFκB p65 BioLegend Cat#653002, RRID:AB_2561613 Anti-NFATc1 BioLegend Cat#649602, RRID:AB_10679126 Anti-mouse IgG HRP-linked antibody CST Cat#7076P2 Anti-rabbit IgG HRP-linked antibody CST Cat#7074S Anti-human CD3 Biocare Cat#CME324B Anti-human CD45RA Invitrogen Life Technologies Cat#14-0458-82, RRID:AB_467272 Anti-human CD8 CST Cat#85336 Anti-human CD4 ABclonal Cat#19018, RRID:AB_2862510 Anti-human CD27 Abcam Cat#Ab131254, RRID:AB_11155136 Anti-human CD45RA Abcam Cat#Ab755, RRID:AB_305970 Chemicals, peptides, and recombinant proteins Human T-Activator CD3/CD28 Invitrogen Cat#11131D Critical commercial assays 123count eBeads Invitrogen Cat#01-1234-42 ELISA MAXTM Deluxe Set Human IFN-γ BioLegend Cat#430104 Luciferase Assay System Promega Cat#E1501 PE Phycoerythrin Fluorescence Quantitation Kit BD Cat#340495 NE-PER Nuclear and Cytoplasmic Extraction Kit ThermoScientific Cat#78833 (Continued on next page) Cell Reports Medicine 6, 102378, October 21, 2025 e1 Article ll OPEN ACCESS ..
Enzyme-linked Immunosorbent Assay:Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cetuximab Lilly NDC Code: 66733-948-23 Goat-anti-human IgG conjugated with PE Jackson ImmunoResearch Cat#109-116-170, RRID:AB_2337681 PE-conjugated anti-MYC mouse monoclonal antibody Cell Signaling Technology (CST) Cat#3739 Anti-human CD3-BV711 BioLegend Cat#317328, RRID:AB_2562907 Anti-human CD3-BV605 BioLegend Cat#317322, RRID:AB_2561911 Anti-human CD62L-APC BioLegend Cat#304810, RRID:AB_314470 Anti-human CD45RA-BV421 BD Cat#569620 Anti-human LAG3-APC BioLegend Cat#369212, RRID:AB_2728373 Anti-human TIM3-PE-Cy7 BioLegend Cat#345014, RRID:AB_2561720 Anti-human PD1-BV421 BioLegend Cat#329920, RRID:AB_10960742 GPC2 (CT3) antibody CST Cat#90488 Anti-Phospho-PLCγ1 CST Cat#2821S Anti-PLCγ1 CST Cat2822S Anti-Phospho-SLP76 CST Cat#14745S Anti-SLP76 CST Cat#4958S Anti-Phospho-LAT CST Cat#3584S Anti-LAT CST Cat#45533S Anti-Phospho-ZAP70 CST Cat#2701S Anti-ZAP70 CST Cat#2705S Anti-Phospho-mTOR CST Cat#5536 Anti-mTOR CST Cat#2983 Anti-GAPDH CST Cat#2118S Anti-Histone H4 CST Cat#2592S Anti-NFκB p65 BioLegend Cat#653002, RRID:AB_2561613 Anti-NFATc1 BioLegend Cat#649602, RRID:AB_10679126 Anti-mouse IgG HRP-linked antibody CST Cat#7076P2 Anti-rabbit IgG HRP-linked antibody CST Cat#7074S Anti-human CD3 Biocare Cat#CME324B Anti-human CD45RA Invitrogen Life Technologies Cat#14-0458-82, RRID:AB_467272 Anti-human CD8 CST Cat#85336 Anti-human CD4 ABclonal Cat#19018, RRID:AB_2862510 Anti-human CD27 Abcam Cat#Ab131254, RRID:AB_11155136 Anti-human CD45RA Abcam Cat#Ab755, RRID:AB_305970 Chemicals, peptides, and recombinant proteins Human T-Activator CD3/CD28 Invitrogen Cat#11131D Critical commercial assays 123count eBeads Invitrogen Cat#01-1234-42 ELISA MAXTM Deluxe Set Human IFN-γ BioLegend Cat#430104 Luciferase Assay System Promega Cat#E1501 PE Phycoerythrin Fluorescence Quantitation Kit BD Cat#340495 NE-PER Nuclear and Cytoplasmic Extraction Kit ThermoScientific Cat#78833 (Continued on next page) Cell Reports Medicine 6, 102378, October 21, 2025 e1 Article ll OPEN ACCESS ..
Luciferase:Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cetuximab Lilly NDC Code: 66733-948-23 Goat-anti-human IgG conjugated with PE Jackson ImmunoResearch Cat#109-116-170, RRID:AB_2337681 PE-conjugated anti-MYC mouse monoclonal antibody Cell Signaling Technology (CST) Cat#3739 Anti-human CD3-BV711 BioLegend Cat#317328, RRID:AB_2562907 Anti-human CD3-BV605 BioLegend Cat#317322, RRID:AB_2561911 Anti-human CD62L-APC BioLegend Cat#304810, RRID:AB_314470 Anti-human CD45RA-BV421 BD Cat#569620 Anti-human LAG3-APC BioLegend Cat#369212, RRID:AB_2728373 Anti-human TIM3-PE-Cy7 BioLegend Cat#345014, RRID:AB_2561720 Anti-human PD1-BV421 BioLegend Cat#329920, RRID:AB_10960742 GPC2 (CT3) antibody CST Cat#90488 Anti-Phospho-PLCγ1 CST Cat#2821S Anti-PLCγ1 CST Cat2822S Anti-Phospho-SLP76 CST Cat#14745S Anti-SLP76 CST Cat#4958S Anti-Phospho-LAT CST Cat#3584S Anti-LAT CST Cat#45533S Anti-Phospho-ZAP70 CST Cat#2701S Anti-ZAP70 CST Cat#2705S Anti-Phospho-mTOR CST Cat#5536 Anti-mTOR CST Cat#2983 Anti-GAPDH CST Cat#2118S Anti-Histone H4 CST Cat#2592S Anti-NFκB p65 BioLegend Cat#653002, RRID:AB_2561613 Anti-NFATc1 BioLegend Cat#649602, RRID:AB_10679126 Anti-mouse IgG HRP-linked antibody CST Cat#7076P2 Anti-rabbit IgG HRP-linked antibody CST Cat#7074S Anti-human CD3 Biocare Cat#CME324B Anti-human CD45RA Invitrogen Life Technologies Cat#14-0458-82, RRID:AB_467272 Anti-human CD8 CST Cat#85336 Anti-human CD4 ABclonal Cat#19018, RRID:AB_2862510 Anti-human CD27 Abcam Cat#Ab131254, RRID:AB_11155136 Anti-human CD45RA Abcam Cat#Ab755, RRID:AB_305970 Chemicals, peptides, and recombinant proteins Human T-Activator CD3/CD28 Invitrogen Cat#11131D Critical commercial assays 123count eBeads Invitrogen Cat#01-1234-42 ELISA MAXTM Deluxe Set Human IFN-γ BioLegend Cat#430104 Luciferase Assay System Promega Cat#E1501 PE Phycoerythrin Fluorescence Quantitation Kit BD Cat#340495 NE-PER Nuclear and Cytoplasmic Extraction Kit ThermoScientific Cat#78833 (Continued on next page) Cell Reports Medicine 6, 102378, October 21, 2025 e1 Article ll OPEN ACCESS ..
Fluorescence:Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cetuximab Lilly NDC Code: 66733-948-23 Goat-anti-human IgG conjugated with PE Jackson ImmunoResearch Cat#109-116-170, RRID:AB_2337681 PE-conjugated anti-MYC mouse monoclonal antibody Cell Signaling Technology (CST) Cat#3739 Anti-human CD3-BV711 BioLegend Cat#317328, RRID:AB_2562907 Anti-human CD3-BV605 BioLegend Cat#317322, RRID:AB_2561911 Anti-human CD62L-APC BioLegend Cat#304810, RRID:AB_314470 Anti-human CD45RA-BV421 BD Cat#569620 Anti-human LAG3-APC BioLegend Cat#369212, RRID:AB_2728373 Anti-human TIM3-PE-Cy7 BioLegend Cat#345014, RRID:AB_2561720 Anti-human PD1-BV421 BioLegend Cat#329920, RRID:AB_10960742 GPC2 (CT3) antibody CST Cat#90488 Anti-Phospho-PLCγ1 CST Cat#2821S Anti-PLCγ1 CST Cat2822S Anti-Phospho-SLP76 CST Cat#14745S Anti-SLP76 CST Cat#4958S Anti-Phospho-LAT CST Cat#3584S Anti-LAT CST Cat#45533S Anti-Phospho-ZAP70 CST Cat#2701S Anti-ZAP70 CST Cat#2705S Anti-Phospho-mTOR CST Cat#5536 Anti-mTOR CST Cat#2983 Anti-GAPDH CST Cat#2118S Anti-Histone H4 CST Cat#2592S Anti-NFκB p65 BioLegend Cat#653002, RRID:AB_2561613 Anti-NFATc1 BioLegend Cat#649602, RRID:AB_10679126 Anti-mouse IgG HRP-linked antibody CST Cat#7076P2 Anti-rabbit IgG HRP-linked antibody CST Cat#7074S Anti-human CD3 Biocare Cat#CME324B Anti-human CD45RA Invitrogen Life Technologies Cat#14-0458-82, RRID:AB_467272 Anti-human CD8 CST Cat#85336 Anti-human CD4 ABclonal Cat#19018, RRID:AB_2862510 Anti-human CD27 Abcam Cat#Ab131254, RRID:AB_11155136 Anti-human CD45RA Abcam Cat#Ab755, RRID:AB_305970 Chemicals, peptides, and recombinant proteins Human T-Activator CD3/CD28 Invitrogen Cat#11131D Critical commercial assays 123count eBeads Invitrogen Cat#01-1234-42 ELISA MAXTM Deluxe Set Human IFN-γ BioLegend Cat#430104 Luciferase Assay System Promega Cat#E1501 PE Phycoerythrin Fluorescence Quantitation Kit BD Cat#340495 NE-PER Nuclear and Cytoplasmic Extraction Kit ThermoScientific Cat#78833 (Continued on next page) Cell Reports Medicine 6, 102378, October 21, 2025 e1 Article ll OPEN ACCESS ..
Quantitation Assay:Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cetuximab Lilly NDC Code: 66733-948-23 Goat-anti-human IgG conjugated with PE Jackson ImmunoResearch Cat#109-116-170, RRID:AB_2337681 PE-conjugated anti-MYC mouse monoclonal antibody Cell Signaling Technology (CST) Cat#3739 Anti-human CD3-BV711 BioLegend Cat#317328, RRID:AB_2562907 Anti-human CD3-BV605 BioLegend Cat#317322, RRID:AB_2561911 Anti-human CD62L-APC BioLegend Cat#304810, RRID:AB_314470 Anti-human CD45RA-BV421 BD Cat#569620 Anti-human LAG3-APC BioLegend Cat#369212, RRID:AB_2728373 Anti-human TIM3-PE-Cy7 BioLegend Cat#345014, RRID:AB_2561720 Anti-human PD1-BV421 BioLegend Cat#329920, RRID:AB_10960742 GPC2 (CT3) antibody CST Cat#90488 Anti-Phospho-PLCγ1 CST Cat#2821S Anti-PLCγ1 CST Cat2822S Anti-Phospho-SLP76 CST Cat#14745S Anti-SLP76 CST Cat#4958S Anti-Phospho-LAT CST Cat#3584S Anti-LAT CST Cat#45533S Anti-Phospho-ZAP70 CST Cat#2701S Anti-ZAP70 CST Cat#2705S Anti-Phospho-mTOR CST Cat#5536 Anti-mTOR CST Cat#2983 Anti-GAPDH CST Cat#2118S Anti-Histone H4 CST Cat#2592S Anti-NFκB p65 BioLegend Cat#653002, RRID:AB_2561613 Anti-NFATc1 BioLegend Cat#649602, RRID:AB_10679126 Anti-mouse IgG HRP-linked antibody CST Cat#7076P2 Anti-rabbit IgG HRP-linked antibody CST Cat#7074S Anti-human CD3 Biocare Cat#CME324B Anti-human CD45RA Invitrogen Life Technologies Cat#14-0458-82, RRID:AB_467272 Anti-human CD8 CST Cat#85336 Anti-human CD4 ABclonal Cat#19018, RRID:AB_2862510 Anti-human CD27 Abcam Cat#Ab131254, RRID:AB_11155136 Anti-human CD45RA Abcam Cat#Ab755, RRID:AB_305970 Chemicals, peptides, and recombinant proteins Human T-Activator CD3/CD28 Invitrogen Cat#11131D Critical commercial assays 123count eBeads Invitrogen Cat#01-1234-42 ELISA MAXTM Deluxe Set Human IFN-γ BioLegend Cat#430104 Luciferase Assay System Promega Cat#E1501 PE Phycoerythrin Fluorescence Quantitation Kit BD Cat#340495 NE-PER Nuclear and Cytoplasmic Extraction Kit ThermoScientific Cat#78833 (Continued on next page) Cell Reports Medicine 6, 102378, October 21, 2025 e1 Article ll OPEN ACCESS ..
Extraction:Article Title: Antibody-gamma/delta T cell receptors targeting GPC2 regress neuroblastoma with low antigen density.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Cetuximab Lilly NDC Code: 66733-948-23 Goat-anti-human IgG conjugated with PE Jackson ImmunoResearch Cat#109-116-170, RRID:AB_2337681 PE-conjugated anti-MYC mouse monoclonal antibody Cell Signaling Technology (CST) Cat#3739 Anti-human CD3-BV711 BioLegend Cat#317328, RRID:AB_2562907 Anti-human CD3-BV605 BioLegend Cat#317322, RRID:AB_2561911 Anti-human CD62L-APC BioLegend Cat#304810, RRID:AB_314470 Anti-human CD45RA-BV421 BD Cat#569620 Anti-human LAG3-APC BioLegend Cat#369212, RRID:AB_2728373 Anti-human TIM3-PE-Cy7 BioLegend Cat#345014, RRID:AB_2561720 Anti-human PD1-BV421 BioLegend Cat#329920, RRID:AB_10960742 GPC2 (CT3) antibody CST Cat#90488 Anti-Phospho-PLCγ1 CST Cat#2821S Anti-PLCγ1 CST Cat2822S Anti-Phospho-SLP76 CST Cat#14745S Anti-SLP76 CST Cat#4958S Anti-Phospho-LAT CST Cat#3584S Anti-LAT CST Cat#45533S Anti-Phospho-ZAP70 CST Cat#2701S Anti-ZAP70 CST Cat#2705S Anti-Phospho-mTOR CST Cat#5536 Anti-mTOR CST Cat#2983 Anti-GAPDH CST Cat#2118S Anti-Histone H4 CST Cat#2592S Anti-NFκB p65 BioLegend Cat#653002, RRID:AB_2561613 Anti-NFATc1 BioLegend Cat#649602, RRID:AB_10679126 Anti-mouse IgG HRP-linked antibody CST Cat#7076P2 Anti-rabbit IgG HRP-linked antibody CST Cat#7074S Anti-human CD3 Biocare Cat#CME324B Anti-human CD45RA Invitrogen Life Technologies Cat#14-0458-82, RRID:AB_467272 Anti-human CD8 CST Cat#85336 Anti-human CD4 ABclonal Cat#19018, RRID:AB_2862510 Anti-human CD27 Abcam Cat#Ab131254, RRID:AB_11155136 Anti-human CD45RA Abcam Cat#Ab755, RRID:AB_305970 Chemicals, peptides, and recombinant proteins Human T-Activator CD3/CD28 Invitrogen Cat#11131D Critical commercial assays 123count eBeads Invitrogen Cat#01-1234-42 ELISA MAXTM Deluxe Set Human IFN-γ BioLegend Cat#430104 Luciferase Assay System Promega Cat#E1501 PE Phycoerythrin Fluorescence Quantitation Kit BD Cat#340495 NE-PER Nuclear and Cytoplasmic Extraction Kit ThermoScientific Cat#78833 (Continued on next page) Cell Reports Medicine 6, 102378, October 21, 2025 e1 Article ll OPEN ACCESS ..
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